Rapid Authentication of Edible Oils by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

摘要

We have developed direct analysis of edible oils using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The data acquisition can be computer-controlled and MALDI-MS spectra of high quality and high reproducibility can be obtained for edible oil samples, and a preliminary spectral database of edible oils has been set up. Since different types of edible oils have different MALDI-MS spectral patterns, the authenticity of an edible oil sample can be determined by comparing its MALDI-MS spectrum with those of its labeled oil in the established database. Our results revealed that mixed edible oils and recycled cooking oils could be screened out from the pure edible oils after the analysis, since heating and mixing of edible oils could change their MALDI-MS spectral patterns. This method is thus capable of rapidly authenticating edible oils and screening out fraudulent mislabeling of edible oils, with a speed of several minutes for one sample.