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Two simple designs for surface-plasmon-resonance-based sensing

机译:基于表面等离子体激元共振的两种简单设计

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Abstract: Surface plasmon resonance spectroscopy is becoming an increasingly important technique in biotechnology and chemical sensing. We present two simple, low cost, high sensitivity devices. The first is laser based mechanical implementation of a Kretschmann setup. Angle sweep is realized in two stages: step motor is used for coarse angle setting, and continuous angle sweep is achieved with a mirror on a floppy disk drive and a cylindrical lens setup. A single detector with AD converter defines resolution of the device through its sampling speed and dynamic range, so high sensitivity can be achieved. Sensor probes are metal- coated microscope slides and sample volume is temperature controlled. Second devices is a disposable cuvette for use in a VIS spectrometer. Specially designed monolithic polycarbonate block provides the required optical path and appropriate incidence angle on a thin metal film, deposited on the block. No equipment is necessary and the cuvette can be used within special cell, such as temperature controlled vessel. Device is also discussed in view of a low cost fiberoptic implementation. Some experimental results are presented to prove the applicability of devices. Disadvantages of technical solutions, used in devices, are also taken into consideration.!22
机译:【摘要】表面等离子体共振光谱学已成为生物技术和化学传感领域越来越重要的技术。我们介绍了两种简单,低成本,高灵敏度的设备。首先是Kretschmann装置的基于激光的机械实现。角度扫描分为两个阶段:步进电机用于粗调角度,而软盘驱动器上的反光镜和柱面透镜设置可实现连续角度扫描。具有AD转换器的单个检测器通过其采样速度和动态范围来定义设备的分辨率,因此可以实现高灵敏度。传感器探头是镀金属的显微镜载玻片,样品量受温度控制。第二个设备是用于VIS光谱仪的一次性比色皿。经过特殊设计的整体式聚碳酸酯块可在沉积在块上的金属薄膜上提供所需的光路和适当的入射角。不需要任何设备,比色皿可以在特殊的室内使用,例如温度可控的容器。鉴于低成本的光纤实现,还讨论了设备。提出了一些实验结果以证明设备的适用性。还考虑了设备中使用的技术解决方案的缺点。!22

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