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【24h】 Simple and Sensitive Protein Detection System using UV LEDs and Liquid Core Waveguides

机译使用UV LED和液芯波导的简单而灵敏的蛋白质检测系统

【摘要】Protein concentrations are traditionally analyzed in the ultraviolet at 280nm using laboratory spectrophotometers. Recently, AlGaN/GaN deep ultraviolet Light Emitting Diodes (LED) became available in the 250 nm to 350 nm wavelength region. An inexpensive fiber optic detection system based on these UV LEDs and photodiodes has been developed. Measurement stability, noise and drift will be discussed. To improve sensitivity of the detection system, microliter volume liquid core waveguide sampling cells with 10 cm and 50 cm pathlength were evaluated. With a continuous flow setup, protein concentrations as low as 1 μg/mL and 0.2 μg/mL were detectable in a 10 cm and a 50 cm pathlength cell respectively, based on a 10 mAU absorbance signal and a signal to noise ratio greater than 10.

【摘要机译】传统上,使用实验室分光光度计在280nm的紫外线下分析蛋白质浓度。最近,AlGaN / GaN深紫外发光二极管(LED)在250 nm至350 nm波长范围内可用。已经开发了基于这些UV LED和光电二极管的廉价光纤检测系统。将讨论测量稳定性,噪声和漂移。为了提高检测系统的灵敏度,对光程为10 cm和50 cm的微升体积液芯波导采样池进行了评估。通过连续流动设置,基于10 mAU吸收信号和大于10的信噪比,分别在10 cm和50 cm光程的池中分别检测到低至1μg/ mL和0.2μg/ mL的蛋白质浓度。 。

【原文格式】PDF

机译 UV-LED;液芯波导;光纤;
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