Real-Time Fluorescence for Determining the Relative Sensitivities of Reactive and Non-Reactive Fluorescent Probes

摘要

The relative sensitivities of structurally similar nitrostilbene based fluorescent probes, bearing substituents of different sizes and reactivities, were determined during the photoinitiated polymerization of HEXDMA by real-time fluorescence spectroscopy. Reactive probes are significantly more sensitive than corresponding non-reactive probes, up to conversions of 78-87%, and the reactivity of the substituent, has a very pronounced effect on the probe sensitivity. Increasing the probe size resulted in a small decrease in probe sensitivity at all stages of the reaction. These findings can be utilized for tuning the sensitivity of fluorescent probes for specific applications.