COMPARISON OF NASAL LAVAGE AND CELL EXPOSURE METHODS IN THE ASSESSMENT OF INFLAMMATORY POTENTIAL OF THE AIRBORNE PARTICLE MATERIAL

摘要

Exposure in moisture and mould damaged indoor environment has been shown to be a potential health risk. We evaluated inflammatory potential of the airborne particle material by measuring the production of proinflammatory cytokines in nasal lavage (NAL) samples of the studied subjects and in cell culture medium of mouse RAW264.7 macrophages which were exposed to the combined extracts of the filter samples representing personal exposure and main microenvironments (home and working place) of the subjects. Cytokine levels in the NAL samples of subjects with high microbial exposure and in the cell culture medium of the macrophages exposed to their corresponding filter samples were increased, as compared to the values of the subjects with low exposure. In conclusion, both methods discriminate between subjects with high and low microbial exposure.