Determination of the Labeling Yield and Stability of the Complexes Bi-BSA-DOTA and Bi-BSA-DTPA

摘要

The procedure using 225Ac/213Bi and 212Pb/212Bigenerators, applied in this study, allows the maximumactivity to be obtained in a volume of 0.5 mL. This, inturn, allows using HFC of type PD MidiTrap G-25, forwhich the recommended volume is 1 mL. The use ofCoomassie dye for protein staining allows calibrationof the GFC and easy determination of the volume inwhich the protein is eluted (Bradford method). Labelingof the conjugate activated with DOTA appeared tobe inefficient because of the formation of more stablecomplexes with other complexing agents present in thesolution. In labeling of the BSA-DTPA conjugate, theyield exceeding 70% was reached. In this case, a sodium citrate solution was used both for pH adjustment and for binding Bi in a complex. This allows simplerand more accurate pH adjustment in the automaticmode. The procedure can be implemented in an automatic synthesis unit.