Characteristic of the kraft mill effluents from Pinus radiata and Eucalyptus globulus by means combination of yeast bioassays and CG-MS chemical analysis

摘要

The goal of this work is to evaluate the characteristic physico-chemical of effluent of Pinus radiata and Eucalyptus globulus used as raw material. These effluents were subjected to biological treatment before their analysis. Solid phase extraction (SPE), are performed with solvents of increasing polarity to determine most of the compounds analyzed through gas chromatography - mass spectrometry (GC-MS). For determine activity of kraft pulp mill effluents (P. radiata, and E. globulus) was evaluated to the Yeast Estrogen Screen (YES) assay, while Ligands of Aryl Hydrocarbon Receptor (AhR) was evaluated by Recombinant Yeast Assay (RYA). By SPE and GC-MS were detected the main groups present in pulp mills effluents, which correspond to fatty acids, hydrocarbons, phenols, sterols and triterpenes. Activity of YES in E. globulus and P. radiata effluents showed values ranked between 1.475 and 0.383 ng/L of EE2 eq. RYA showed an effective concentration of AhR ligands significantly higher E. globulus than in P. radiata effluents. Analysis of their respective composition by SPE and GC-MS, suggested that aromatic compounds were the main determinants activity in the samples. In particular, benzaldehyde derivatives appeared as responsible for the activity, due to their structural properties and their high concentration in AhR ligand-rich samples.