Facile hydrothermal synthesis of silver nanoparticles using holocellulose as substrate and reducing agent and their antibacterial activity

摘要

In this study,the silver nanoparticles(AgNPs)with relatively uniform size and well-defined structure have been synthesized by using a hydrothermal method with holocellulose(HC)as substrate and reducing agent for silver ions.This method was simple,cost effective and convenient to handle without usage of any other stabilizing or reducing agents.The experimental results showed that the size,morphology and contents of AgNPs in the HC/Ag nanocomposites could be regulated by adjusting the hydrothermal time.The as-prepared HC/Ag nanocomposites exhibited good cytocompatibility to the cells and highly toxic to microorganisms.The antibacterial activity studies were carried out on both Gram-positive S.aureus and Gram-negative E.coli bacteria by disc diffusion method and agar dilution method.The important role of HC and the influence of the additional reducing agents(NaBH4 and ascorbic acid)on the products were systematically investigated,and the formation mechanism of HC/Ag nanocomposites was proposed.These results indicate that HC was a good candidate for the preparation of silver-based nanocomposites,and the as-prepared HC/Ag nanocomposites with good cytocompatibility and excellent antibacterial activity may have potential for use in a wide range of biomedical applications.