COMPARATIVE STUDY OF Al-AND Zn-PHTHALOCYANINES UPTAKE IN RABBIT ILIAC ARTERY BY TRANSADVENTITIAL MEASUREMENTS OF INDUCED FLUORESCENCE

摘要

Comparative studies were carried out on the Al-phthalocyanine (AlPc) and Zn-phthalocyanine (ZnPc) uptake in iliac artery wall of rabbit, through transadventitial fluorescence measurements. Two 3.5 Kg Norfolk male rabbit were used. The iliac artery was dissected, isolated and clamped at two points, 1.5 cm apart. The experiment comprised three steps, collecting fluorescence spectra at several points inside and outside of the clamped region of the artery. The excitation source was a 488 nm Argon Laser. Firstly, it was measured the pre and post clamping transadventitial fluorescence without the photosensitizer. After that, 50 μL of AlPc or ZnPc solutions in Cremophor EL, 2.5 μM, were injected locally into the artery clamped region of each rabbit and fluorescence spectra were collected at t=0 and 15 min. Finally, elapsed 30 min the clamping was removed, restarting the blood flow through the artery, and more spectra were collected at t=15 and 30 min. Temporal evolution of the drug uptake by the artery wall was studied analyzing the intensities of the fluorescence peaks at 682 and 678 nm for AlPc and ZnPc, respectively. It was found that AlPc is more absorbed and remains for a longer time in the iliac artery wall than the ZnPc.