SOLID SUPPORTED BILAYERS ON SAM FUNCTIONALISED SUPPORTS

摘要

The unique selectivity found in naturally occuring biological systems offers great potential for the development of sensors with both high selectivity and sensitivity. Since most systems rely on the utilisation of membrane proteins the attachment of phospholipid bilayers to solid supports represents an important goal in the development of a range on biological sensors. In the work presented here we show how self-assembled monolayers can be utilised in the attachment of such bilayers to solid supports. The bilayer formation, via vesicle fusion with a suitably modified surface, was followed using SPR and Impedance analysis. The attached bilayers were then characterised using FTIR-ATR, CFM and AFM. Finally, we show that the channel forming protein Gramicidin and the ion- transporter Valinomycin display the expected ion transport behaviour.