Use of Fatty Acid Profiles To Identify White-Rot Wood Decay Fungi

摘要

Identification libraries have been created for the white-rot fungi Phanerochaete chrysosporium, P. sordida , P. sanguinea, Trametes versicolor, T. hirsuta, and T. pubescens. Libraries were compiled using five to ten isolates of each species with each isolate cultured four times, extracted in duplicate and analyzed by gas chromatography for its fatty acid methyl ester (FAME) profile. Single libraries were created for T. versicolor and T. pubescens, indicating that all isolates of these species clustered as a single species. More than one library was created for P. chrysosporium, P. sordida, and T. hirsuta, indicating that not all isolates of these species clustered as a single species. The P. chrysosporium libraries provided a good identification of all but one blind sample. Analysis of that sample identified P. sordida as first choice, but the second choice of P. chrysosporium was too close for a good species match. The genus Phanerochaete was correctly identified in all blind samples for both species tested. Blind samples of T. pubescens and T. hirsuta were not distinguished at the species level. There appears to be considerable variability within T. hirsuta. It will be interesting in the future to compare the DNA sequences of these outliers to determine if the genetic information supports the separations seen in the FAME profiles.