Determination of affinity and kinetic constants of the biotin-streptavidin complex using GMR microfluidic biosensor

摘要

Studies of the interactions between biological molecules are of highest importance in a vast number of areas of biomedicine and biotechnology. Today there are several different methods used for affinity studies such as surface film resonance, mass spectroscopy, ultracentrifugation, chromatography and electrophoresis and so on. Our approach utilizes GMR microfluidic biosensor, an emerging idea tool for biomolecules detection applications. The GMR biochip combined with microfluidic system has higher reaction efficiency. The GMR microfluidic biosensor has been successfully used to perform online kinetic measurements of biotin and streptavidin. The affinity and dissociation reaction rate constants are consistent with literature results.