Effect on the Boar Sperm Motility PRRS Virus

摘要

The objective of the study was to evaluate the sperm viability of semen infected with PRRS viral particles and the effect of the Virus on the mobility of boar spermatozoa.The work was carried out in the laboratory of genetics and reproduction FMVZ-BUAP.Semen was obtained from the center of Insemination in Tecamachalco.Puebla.5 Stallions were used.Each sample containing 1×106 spermatozoa,PRRS virus strain was ATCC-VR-2332(0,102,104 and 106 prints RNA/mL in triplicate),observed daily in the home ; Hamilton Thorne(R).Cells with Total Motility(MT)(P＜0.05)on days 1,3,5,7 and 10 of the evaluation.Curvilinear Velocity(VCL)on day I and 7 to 10 x 10 x6 and 2 PI(P＜0.05 )vs control.Velocity Straight Line(VSL)and Velocity Average Path(VAP)in day 1 day Postinfection(DPI)10 ×2,10×4 and 10×6 ; (P＜0.05)yS.the control.The Amplitude of the Lateral Head(ALH)on day 5 and 7 to 10×4 and 10×6 DPI(P＜0.05)vs control.The Progressive Motility(MP)on day 1 and 3 DPI to 10 ×10×4 and 2(P＜0.05 )vs control.In the matrix of correlations,observed a correlation(P≥0.001 )between VSL with VAP; VSL with VCL.Also between VCL and ALH.The VAP with ALH.The VSL with HLA ; the STR with ALH and VCL str.Finally,found a relationship between the different types of sperm mobility arising out of the House,resulting in that the addition of PRRS Virus in boar semen affects the motility of sperm.