OBSERVATIONS OF PROLIFERATION AND DIVISION OF FROZENTHAWED MESENCHYMAL STEM CELLS (MSCS) USING A TIME-LAPSE CINEMICROSCOPY

摘要

Cryopreservation and culture of human cells and tissues are indispensable techniques not only for thernregenerative medicine but also for the conservation of gene resources. The cinemicroscope recording of therncell-lysis during freezing and thawing process and of the cell proliferation in the culture dishes in 5 % CO_2 atrn37 ℃ has played an important role superior to still images. We have previously elucidated the mechanismsrnof cryo-destruction and -protection of the human blood and tissue cells using cryomicroscopic observation,rnwhich were recorded by a high-speed 16 mm movie camera since 1967 (Sumida, 1973; 1974; 2016).rnRecently, we made the time-lapse cinemicroscope recording of proliferation, differentiation, and division ofrnmesenchymal stromal cells (MSC) derived from frozen-thawed adult human adipose tissue in a speciallyrndesigned small incubator sized of 25×85×125 mm (BlAST Inc. Japan) for cell and tissue culture which wasrnincorporated on the stage of inverted microscope Nikon DIAPHOT-TMD, using NIKON D800 camera.rnThese results will help to elucidate the mysterious behaviour of MSCs, which have solved some problems inrnclinical use of human MSCs, artificially induced on plastic surfaces, in regenerative medicine (Sumida,rn2016).