In Vitro Test and Application for Guided Bone Regeneration of beta-Tricalcium Phosphate / Poly-(Lactide-Glycolic Acid-epsilon-Caprolactone) Composites

摘要

In order to realize easy handling films, novel composites of beta-tricalcium phosphate (TCP) and poly-(lactide-glycolic acid-epsilon-caprolactone) (PLGC) having a softening temperature of about 40 deg C were prepared by a heat-kneading method. The composite prepared could be easily formed into a cylindrical membrane at 40 deg C, and its tensile strength was greater than that of a pure PLGC. From Fourier-transformed infrared spectroscopy, it was shown that the chemical interaction formed between TCP and PLGC. Physiological saline soaking test indicated that TCP inhibited hydrolysis of PLGC by auto-controlling saline pH, resulting in almost constant tensile strength. Mandibular 2-wall bone defects 2x1x1cm~3 in size and tibia fully defects 2cm in length of beagles were filled with new bone 12 weeks after guided bone regeneration (GBR) operation using the composite membrane, although a pure PLGC membrane could not repair such defects. The composite membrane was useful for the GBR membrane.