Incorporation of Proteins into Biomimetic Hydroxyapatite Coatings

摘要

Hydroxyapatite coating was biomimetically deposited on titanium alloy (Ti6Al4V). Various concentrations (10ng/ml - 1mug/ml) of bovine serum albumin (BSA) were added into a supersaturated calcium phosphate solution (CPS) at physiological temperature and pH of 7.4. Pre-treated Ti6Al4V plates were immersed into such solution for 48 hours at 37 deg C. BSA was co-precipitated with the crystals during the coating process. A white and thick (30 - 50mum) coating was uniformly deposited on titanium surfaces. The produced coatings were evaluated and protein release was measured. Results revealed: at higher BSA concentrations in the solution, the coating changed its microstructure; the crystal size of the coating and the coating thickness decreased indicating a crystal growth inhibition. Loading amounts of protein in the coating increased with higher concentration in the solution. Protein was incorporated into whole layer of coating and lead to a slow release. These results indicated that biomimetic hydroxyapatite coatings are suitable carriers for proteins.