INTERACTION OF FIREFLY LUCIFERASE LUCIOLA MINGRELICA WITH DIMETHYLOXYLUCIFERIN

摘要

Oxyluciferin in its singlet electronically excited state is the product of the firefly bioluminescent reaction. The examination of its fluorescent properties in solution and in complexes with firefly luciferase provides useful information about the mechanism of firefly bioluminescence. Dimethyloxyluciferin (DMOL) is an oxyluciferin analog (Fig. 1), that exists only in a keto form, whereas oxyluciferin can be in the keto or enol form. That is why it was interesting to study the spectral and fluorescent properties of DMOL and its interaction with firefly luciferase.