Calcium Level-Responsive Zinc Ion Release from Tricalcium Phosphate Containing Zinc (ZnTCP)

摘要

Tricalcium phosphate containing Zn (ZnTCP; 0.63, 6.17 and 12.05 Zn w/wpercent) were investigated Zn~(2+) release from ZnTCP. The in-vitro release rates from Zn-TCP powders were measured in 25 ml of SBF containing 10 mg/100mL Ca~(2+)(SBF (H)), SBF containing 5 mg/100mL Ca~(2+) (SBF(L)) or SBF without Ca~(2+) (SBF(-)) at pH7.25, 37.0+0.1 deg C. The Zn~(2+) release rate from 6 and 12percent ZnTCP were much faster than that at the latter stage. The Zn~(2+) release rate from ZnTCP decreased with increasing Ca~(2+) concentration in dissolution media. The rate from ZnTCP increased with increasing Zn~(2+) concentration in TCP. On the other hand, the aqueous ZnTCP suspension containing 10mg of 6 and 12percent ZnTCP was subcutaneously injected into the backs of the ovarictomized female Wistar rats. The plasma Zn~(2+) levels at 1-5 days of ZnTCP loaded rats increased comparing with the control disease model. The area under the curve of Zn release profiles (AUC) of 6 and 12percentZnTCP were significantly higher than that of control disease model.