壮药喙荚云实的抗肿瘤化学成分及其作用靶标的研究

摘要

目的:研究喙荚云实的抗肿瘤活性成分.方法:采用柱色谱技术对喙荚云实提取物进行分离纯化,用波谱技术进行结构鉴定,结合计算机虚拟筛选技术寻找作用靶标.结果:从喙荚云实中分离得到了37个卡山烷类化合物, 分别鉴定为:1α,6α-diacetoxy-5α-hydroxy-15-carboxymethyl-12-oxocassa-7(8),13(14)-diene(1)、lα,6α,7β-triacetoxy-5α-hydroxy-15-carboxymethyl-12-oxocassa-13(14)-diene(2)、lα,6α,7β-triacetoxy-5α-hydroxy-16,16-dimethoxy-12-oxocassa-13(14)-diene(3)、1α, 6α,7β-triacetoxy-5α-hydroxy-13-aldehyde-14-methylcassa-13(14)-diene (4) 、1 α-acetoxy-7-oxo-14-methylvoucapane-5(6),8(14),9(11)-diene(5)、lα,6α-diacetoxy-5α,7β-dihydroxyl-14-methylvoucapane-8(14),9(ll)-diene(6)、lα,6α,7β-triacetoxy-5α-hydroxyl-14-methylvoucapane-8(14),9(11)-diene(7)、1α,6α-diacetoxy-5α,7β-dihydroxyl-12α-methoxycassa-14(17),13(15)-diene-16,12-olide(8)、lα-acetoxy-5α,7β-dihydroxyl-12α-methoxycassa-14(17),13(15)-diene-16,12-olide(9)、lα,6α,7β-triacetoxy-5 α-hydroxyl-14α-methylolcassa-11(12),13(15)-diene-16,12-olide(10)、lα,2α-diacetoxy-5α-hydroxyl-12α-methoxy-14α-methylcassa-13 (15)-en-16,12-olide(11)、lα,7β-diacetoxy-5α-hydroxyl-12α-methoxy-14α-carboxymethyl cassa-13 (15)-en-16,12-olide(12)、lα,6α-diacetoxy-5α,7β,12α,14α-tetrahydroxyl-14β-methylcassa-13(15)-en-16,12-olide (13)、5α-(N-methyl)methoxy-lα,2α-diacetoxy-14-methylcassa-8(9),11(12),13(14)-diene-16,12-olide(14)、lα,6α,7β-triacetoxy-5α,13α,15β,16β-tetrahydroxy-12-oxocassane-14,16-epoxy(15)、Neocaesalpin MO(16)、7β-acetoxy-6β-hydroxyneocaesalpin E(17)、6β-hydroxyne10ocaesalpin E(18)、Neocaesalpin W(19)、Neocaesalpin C(20)、Neocaesalpin J(21)、12,16-epoxy-5 α-hydroxy-12α-methoxycassa-13-(15)-en-16-one(22)、Neocaesalpin D(23)、Neocaesalpin N(24)、12α-ethoxyl-lα,6α,7β-triacetoxy-5α,14β-dihydroxy-cass-13(15)-en-16,12-olide (25) 、lα,7β-diacetoxy-5α,12α-dihydroxy-cass-13(15)-en-16,12-olide-17β-carboxylate (26)、Daucosterol (27)、Neocaesalpin I(28)、Neocaesalpin MA (29)、TaepeeninF(30)、Norcaesalpinin C(31)、Taepeenin K(32)、Taepeenin L (33)、Taepeenin G(34)、Chagresnol (35)、6β,18-diacetoxycassan-13,15-diene (36)、Caesalpinin ME (37)。结论：化合物1-15为新化合物.