Experiment of transplanting Metanephric mesenchymal transfected by uPA gene to therapy chronic kidney disease

摘要

Objective Study regeneration role and possible mechanism of metanephric mesenchymal and metanephric mesenchymal transfected by uPA gene in CKD.Method 1. Construction and package of recombinant plasmid of uPA (urokinase-type plasminogen activator) gene and pAAV-IRES- hrGFP vector.2. The viral particles of rAAV and AAV were transfected into rat's metanephric mesenchymal (rat-inducible metanephric mesenchymal-18, RIMM-18) in vitro and the positive RIMM-18 cells that expressed GFP were detected using the inverted fluorescence microscope.3.Study regeneration role and possible mechanism ofmetanephric mesenchymal and metanephric mesenchymal transfected by uPA gene in CKD that were transplanted via caudal vein.