Clinicopathological significance of miR-10b expres

摘要

OBJECTIVE：To investigate microRNA expression profiles of gastric cancer, to explore miR-10bexpression in human gastric cancer cell lines, non-malignant gastric epithelial cell line GES-1 andgastric cancer tissues, and to determine the effect of miR-10b overexpression on cancer cellproliferation, migration, invasion and Hoxd10 expression.DESIGN：miRCURYTM LNA Arrays (v.16.0) were used to screen miRNAs in 17 gastric cancer.RT-PCR was performed to determine the expression of miR-10b in 56 gastric tumours and gastriccancer cell lines. The effect of miR-10b on proliferation, migration and invasion of MKN-28,BGC-823 and SGC-7901 cells, and the expression of HOXD10 protein in SGC-7901 andBGC-823 cells were detected following transfection of miR-10b paraffin-embedded cancer tissueswas also investigated. inhibitor or Negative Control B. Expression of miR-10b and HOXD10 protein in 436.