METHOD FOR DISCRIMINATING BETWEEN LIVE AND DEAD MICROBES IN A SAMPLE

摘要

The present invention relates to a method for discriminating between live and dead microbes in a sample, by discriminating between transcriptionally-active and inert microbial nucleic acid sequences in the sample. In particular, the method according to the present invention is based on the comparison of levels of nucleotide substitution in a sample cultured in presence of an RNA-labelling agent.The present invention further relates to a diagnosis method of microbial infections in a subject; and to methods of assessing the risk of contamination of a sample, implementing the method for discriminating according to the present invention.