A STRIP TEST FOR DETECTING ENZOOTIC BOVINE LEUKOSIS AND A METHOD FOR DETECTING ENZOOTIC BOVINE LEUKOSIS WITH THE USE OF THE STRIP TEST

摘要

The object of the invention is a strip test for detecting enzootic bovine leukosis by assaying anti-BLV antibodies in a sample, characterized in that it contains the gp51 antigen obtained in a baculovirus system and immobilized on a nitrocellulose membrane and A, G, A/G proteins labelled with coloured nanoparticles, as well as glass fibre filter paper for filtering a sample, wherein the analysed sample is cow's milk. A further object of the invention is a method for detecting enzootic bovine leukosis with the use of the strip test according to the invention, comprising the following steps: a. A milk sample is diluted three times with 20 mm Tris-HCI buffer pH 7.5 containing 0.3 % casein and 0.15%Tween 20; b. The milk sample is instilled into the window on the test cassette and incubated for 10 minutes; c. A test result is read in the test window, one bar or spot indicating a negative result and two bars or spots indicating a positive result.