METHOD FOR ASSESSING THE REPRODUCTIVE QUALITIES OF SIRES BY THE CONTENT OF PHOSPHORUS AND SELENIUM IN SEMINAL PLASMA

摘要

FIELD: animal husbandry.;SUBSTANCE: invention relates to the field of animal husbandry, in particular to cattle breeding, and can be used to assess the reproductive qualities of sires. The method for assessing the reproductive qualities of sires includes the selection of fresh semen with a volume of at least 2 ml. Next, the seminal plasma is separated by centrifuging the samples at 400 g for 5 minutes. The concentration of phosphorus and selenium in the seminal plasma is determined by the methods of atomic emission and mass spectrometry with inductively coupled plasma, followed by the calculation of the sperm quality factor (Csf) according to the formula: Csf = phosphorus * selenium, where Csf is the sperm quality factor, conventional units; phosphorus is the concentration of phosphorus in seminal fluid, mcg/g; selenium - the concentration of selenium in the seminal fluid, mcg/g. With a Csf value less than or equal to 943.3 conventional units, the reproductive qualities of the sire are assessed as low, in the range of 943.4–1998.8 conventional units as medium, with a coefficient value greater than or equal to 1998.9 conventional units, as high.;EFFECT: invention provides an effective method for assessing the reproductive qualities of sires.;1 cl, 1 tbl, 3 ex