Induction of pancreatic β cells by stem cell differentiation with RNA

摘要

PROBLEM TO BE SOLVED: To provide a novel method for inducing or generating pancreatic β cells from human induced pluripotent stem cells with unprecedented efficiency and functionality. The core of the present invention is at a number of definitive differentiation determinants in a previously unknown manner, along the pathway from pluripotency to endoderm to endoderm to pancreatic endoderm to pancreatic β-cells. The use of mRNA discovered by experiments. The present disclosure discloses by regulating cell growth kinetics and related parameters, whereby specific combinations of cell density, reagent concentration, and mRNA combinations are used to control differentiation / induction orientation). , Provide methods for inducing stem cell differentiation. [Selection diagram] None