THREE-DIMENSIONAL IN VITRO ALVEOLAR LUNG MODEL, METHOD OF OBTAINING THIS MODEL AND ITS APPLICATION FOR DETERMINING AND/OR PREDICTING THE SENSITIZING EFFECT OF INHALED PRODUCTS

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology, namely to a three-dimensional model of the alveolar lung in vitro, containing essentially the following four types of cells: type II alveolocytes (lining the lung) capable of secreting a surfactant, endothelial cells that form the inner membrane of the capillaries, providing a permeable barrier, dendritic cells , such as undifferentiated THP-1, combining innate and acquired immunity, and macrophage-like cells that can take part in protective reactions through the absorption of foreign substances by phagocytosis. A method is proposed for obtaining this model and its application for assessing the potential ability of inhaled products, such as particles or molecules, to cause irritation of the air-blood barrier of the lungs or for assessing the toxic effect of inhaled products, such as particles or molecules, on the air-blood barrier of the lungs, and also for determining the sensitizing effect. inhaled products, such as particles or molecules, on the air-blood barrier of the lungs.;EFFECT: in vitro method can be used as an alternative to in vivo testing, which allows the identification and characterization of the ability of chemicals to act as sensitizers.;16 cl, 2 tbl, 22 dwg, 3 ex