IDENTIFICATION OF PROTHROMBOTIC CONDITIONS

摘要

Disclosed herein is a method of diagnosing an immune-mediated or inflammatory prothrombotic condition in a subject, the method comprising: a. Combining a sample of plasma obtained from the subject with a sample of whole blood obtained from a healthy donor to obtain a combined sample, b. Contacting the combined sample with at least one agonist to obtain a test sample, c. Determining the proportion of procoagulant platelets in the test sample and in at least one control sample, said determining comprising: i. Contacting the test sample and the at least one control sample with GSAO and an alpha granule detection agent, wherein platelets in the test sample and the at least one control sample which have both increased uptake of GSAO and increased surface expression of alpha granule protein compared to an assay control sample are identified as procoagulant platelets, and d. Determining whether the subject is suffering from the immune-mediated or inflammatory prothrombotic condition, wherein an increased proportion of procoagulant platelets in the test sample compared to the at least one control sample indicates that the subject is suffering from the immune-mediated or inflammatory prothrombotic condition.