首页> 外国专利> CULTURE MEDIUM FOR PRODUCING EXTRACELLULAR VESICLES, CULTURE MEDIUM KIT, ADDITIVE, AND METHOD FOR PRODUCING EXTRACELLULAR VESICLES

CULTURE MEDIUM FOR PRODUCING EXTRACELLULAR VESICLES, CULTURE MEDIUM KIT, ADDITIVE, AND METHOD FOR PRODUCING EXTRACELLULAR VESICLES

机译:生产细胞外小泡的培养基,培养基试剂盒,添加剂,和生产细胞外小泡的方法

摘要

The present invention addresses the problem of providing a culture medium for producing extracellular vesicles, a culture medium kit, an additive, and a method for producing extracellular vesicles, whereby the production of extracellular vesicles is facilitated. The present invention provides a culture medium for producing extracellular vesicles, the culture medium comprising: a basal culture medium for culturing animal cells; and at least one component selected from L-glutamine or a salt thereof, transferrin, selenous acid or a salt thereof, insulin, an insulin-like growth factor, a serotonin compound, and transforming growth factor β. If the culture medium for producing extracellular vesicles contains L-glutamine or a salt thereof, the concentration of the L-glutamine or salt thereof is 5 mM or greater. The present invention also provides a culture medium kit, an additive, and a method for producing extracellular vesicles.
机译:本发明解决了提供用于产生细胞外小泡的培养基、培养基试剂盒、添加剂和产生细胞外小泡的方法的问题,由此促进了细胞外小泡的产生。本发明提供了一种用于产生细胞外囊泡的培养基,该培养基包括:用于培养动物细胞的基础培养基;以及至少一种选自L-谷氨酰胺或其盐、转铁蛋白、亚硒酸或其盐、胰岛素、胰岛素样生长因子、血清素化合物和转化生长因子β的成分。如果用于产生细胞外小泡的培养基含有L-谷氨酰胺或其盐,则L-谷氨酰胺或其盐的浓度为5 mM或更高。本发明还提供一种培养基试剂盒、添加剂和用于产生细胞外囊泡的方法。

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