The present invention relates to a method for producing a SlMS10 ( Solanum lycopersicum male sterile 10) gene knockout tomato plant using a CRISPR/Cas9 system and a male sterile tomato plant prepared by the method, and the present invention relates to male sterility through the CRISPR/Cas9 system A tomato plant was prepared, which can be quickly introduced into an elite line, and has the advantage of reducing the time required to remove linkage drag compared to the existing breeding method.
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