MINIATURIZED, LOW-COST, AND MULTI-CONTRAST UNMARKED MICROSCOPIC IMAGING SYSTEM

摘要

The present invention discloses a miniaturized, low-cost, and multi-contrast unmarked microscopic imaging system. The imaging system is based on an inverted microscopic structure, a highly integrated optical system is designed by adopting a micro lens having a fixed focal length, and a complex optical system of a traditional microscope system is replaced, such that the whole microscope is highly integrated. The system uses a programmable LED array as an illumination light source; the LED array is controlled by a computer to display different illumination modes; six imaging functions of a bright field, a dark field, a rainbow dark field, Rheinberg optical dyeing, differential phase contrast, and quantitative phase imaging are achieved; and diversified unmarked imaging methods are provided for biological applications. The present invention provides a matching control system, which can realize system hardware control and algorithm execution and display, comprises functions such as illumination control, camera parameter adjustment, quantitative phase reconstruction recovery, two-dimensional/three-dimensional result display, and quantitative profile analysis, and can realize diversified information obtaining and analysis of unmarked samples.