Oxidase-Based Chemiluminescence Assay of Phagocytic Leukocytes from Whole Blood and Body Fluids Applicable for Point-of-Care (POC) Measurement of Point-of-Care (POC) Diagnostic Tests of Absolute Neutrophil Function (ANF)

摘要

stimulates NADPH oxidase activity of phagocytes; A method for estimating the number of phagocytes in a bodily fluid of an animal comprising quantifying the generated reductive deoxygenation of a chemiluminescent substrate by the emitted chemiluminescence of the chemiluminescent substrate using an instrument capable of measuring light. The NADPH oxidase activity of phagocytes is preferably stimulated by an immunological or chemical agent capable of activating a respiratory burst by the phagocyte, and by an irritant coated in solution or on a surface in contact with the phagocyte. The irritant is preferably phorbol myristate acetate (PMA). The animal is preferably a human, and the bodily fluid is preferably blood and/or spinal fluid. The phagocyte is preferably a neutrophil leukocyte, and the chemiluminescent substrate is preferably lucigenin (N,N'-dimethyl-9,9'-biacridinium dinitrate). Also provided is a method of treatment based on a presumptive method.