GENETIC CONSTRUCT FOR GENE EXPRESSION mNG_CD4-CXCR4, RECOMBINANT PLASMID rVSV_MNG_CD4-CXCR4 AND A RECOMBINANT VESICULAR STOMATITIS VIRUS STRAIN rVSV_mNG_CD4-CXCR4, WHICH PROVIDES TARGETED VIROLYSIS OF CELLS EXPOSING HIV-1 GP120/GP41 PROTEINS TROPISM X4 ON THEIR SURFACE

摘要

FIELD: genetics.;SUBSTANCE: group of inventions relates to a genetic construct for mNG_CD4-CXCR4 gene expression, a recombinant plasmid coding a cDNA copy of the genome of a replication-defective vesicular stomatitis virus, and recombinant vesicular stomatitis virus exhibiting human CD4 and CXCR4 receptors on its surface. Disclosed is a genetic construct for mNG_CD4 and CXCR4 gene expression, formed by sequences of fluorescent protein mNeonGreen and a human CD4 receptor and CXCR4 coreceptor fused through a P2A peptide and having the nucleotide sequence SEQ ID NO: 1. Disclosed is a recombinant plasmid rVSV_mNeonGreen [CD4-CXCR4], which codes human CD4 and CXCR4 receptors as part of a cDNA copy of the genome of the recombinant vesicular stomatitis virus (VSV), having size of 15260 bp, and containing said genetic construct. Also disclosed is a recombinant strain rVSV_mNG_CD4-CXCR4, containing said recombinant plasmid and providing targeted virolysis of cells, exposing HIV-1 tropism X4 gp120/gp41 proteins on their surface. Strain is deposited in the State collection of agents of viral infections, rickettsial infections of the Federal State Budgetary Institution State Science Center Vector of Federal Service for Supervision of Consumer Rights and Human Welfare, No. V-1108.;EFFECT: group of inventions provides a wider range of genetic structures for mNG_CD4-CXCR4 gene expression, recombinant plasmids and recombinant replication-defective viruses providing virolysis of cells exhibiting gp120/gp41 HIV-1 tropism X4 on their surface.;3 cl, 8 dwg, 4 tbl, 9 ex