PCR primer sets and adapters for amplifying and sequencing mitochondrial ribosomal RNA gene used for DNA barcoding and metabarcoding of fishes

摘要

The present invention performs a polymerase chain reaction (PCR) amplification reaction using genomic DNA or environmental DNA in order to perform DNA barcoding and metabarcoding for fish, and DNA base It relates to novel primer combinations and adapters for deciphering the sequence, and more specifically, performing a PCR amplification reaction of the fish mitochondrial 16S ribosomal RNA (16S rRNA) gene (16S rDNA) and decoding the DNA sequence It relates to novel primer combinations and adapters for The present invention can facilitate the identification of various fish species by increasing the success rate and efficiency of the PCR amplification reaction, and thus is usefully used for molecular phylogenetic and ecological studies of fish.