The mass production process of teriparatide using E.coli

摘要

The present invention relates to a method for mass production of teriparatide using a teriparatide fusion protein, adsorption chromatography, and a Tobacco etch virus (TEV) protease. In addition, the present invention provides a teriparatide fusion protein encoded by the nucleotide sequence of SEQ ID NO: 1; a teriparatide fusion protein expression vector comprising the sequence; And it relates to a transformant into which the expression vector is introduced. When the production method of the present invention is used, it is more economical than the existing production method of teriparatide and shows the same or superior purification effect, so teriparatide with high purity can be obtained, and it is useful industrially as a mass production method it could be