The present invention discloses an accurate and reliable method for early detection and follow-up of breast cancer in a subject, with remarkable sensitivity and specificity along with minimal false results. The present invention also discloses a multianalyte biomarker panel for accurate and reliable method of early detection and follow-up of breast cancer in a subject with remarkable sensitivity and specificity along with minimal false results. The biomarker panel encompasses characterized three methylation biomarkers of genes comprising of SOSTDC1, WIF1, DACT2
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