A SYSTEM AND A METHOD FOR THE DIAGNOSIS OF AN INFECTIOUS DISEASE

摘要

The present invention provides a system and a method for the diagnosis of an infectious disease by a novel nested loop-mediated isothermal amplification method with higher sensitivity and higher specificity. The system includes the designing of a plurality of primers based on the plurality of target regions of the target genome. The method employs a technique of reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP), loop-mediated isothermal amplification (LAMP) and nested loop-mediated isothermal amplification (LAMP). The present method provides the system and the method for the diagnosis of an infectious disease from a small microbial load, thereby allowing diagnosis at an early stage of infection.