The present invention relates to a bio-surfactant production apparatus having first and second air injection tubes, a foam sensing unit and a foam discharge unit for concentrating bubbles and recovering a culture solution, wherein the bio-surfactant production apparatus includes a foam sensing unit; By providing the first and second air inlet tubes, highly concentrated bubbles can be obtained by controlling the amount and concentration of bubbles, and by providing a bubble discharge part, bubbles generated during culturing microorganisms overflow or the culture solution is discharged together with bubbles This can reduce the loss of culture medium. In addition, when culturing microorganisms using the bio-surfactant production apparatus according to the present invention, it is possible to protect the culture, and by removing some cells present in the collected foam, it is possible to extend the period for producing the surfactant. It is possible to improve the production efficiency of biosurfactants.
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