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THREE-DIMENSIONAL MICRO-ENVIRONMENT STRUCTURE FOR CONTROLLING CELL BEHAVIOR, THREE-DIMENSIONAL SURFACE FOR CONTROLLING CELL BEHAVIOR, AND METHOD FOR MANUFACTURING ARRAY AND THREE-DIMENSIONAL MICRO-ENVIRONMENT STRUCTURE
THREE-DIMENSIONAL MICRO-ENVIRONMENT STRUCTURE FOR CONTROLLING CELL BEHAVIOR, THREE-DIMENSIONAL SURFACE FOR CONTROLLING CELL BEHAVIOR, AND METHOD FOR MANUFACTURING ARRAY AND THREE-DIMENSIONAL MICRO-ENVIRONMENT STRUCTURE
The present invention is a nanofiber membrane; and P1 (first peptide) and P2 (second peptide) as a three-dimensional microenvironment structure for regulating the behavior of human induced pluripotent stem cells comprising two or more peptide motifs, wherein the nanofiber membrane is polyvinylidene (PVDF), poly It contains at least one of acrylonitrile (PAN), polyethersulfone (PES) and polylactic acid (PLA), wherein the P1 (first peptide) motif binds to integrin α5β1 As an Integrin binding motif, PHSRN- It contains the amino acid sequence of RGDSP (SEQ ID NO: 17), wherein the P2 (second peptide) motif consists of a Frizzled receptor, a Transforming growth factor (TGF) receptor, a Fibroblast growth factor (FGF) receptor, and a Leukemia inhibitory factor (LIF) receptor. One or more integrin α5β1 co-receptor-binding motifs selected from the group (co-receptor binding mo-tif), and the P2 (second peptide) motif binding to the Frizzled receptor is LCCGRGHRTRTQRVTERCNC (SEQ ID NO: 39) and LGTQGRLCNKTSEGMDGCEL ( SEQ ID NO: 40) is at least one amino acid sequence selected from, and the P2 (second peptide) motif binding to the TGF receptor is at least one amino acid sequence selected from LTGKNFPMFHRN (SEQ ID NO: 37) and MHRMPSFLPTTL (SEQ ID NO: 38), The P2 (second peptide) motif binding to the FGF receptor is at least one selected from the group consisting of ANRYLAMKEDGRLLAS (SEQ ID NO: 33), HFKDPKRLYCK (SEQ ID NO: 34), FLPMSAKS (SEQ ID NO: 35) and KTGPGQKA (SEQ ID NO: 36) an amino acid sequence, and the P2 (second peptide) motif binding to the LIF receptor is IVPLLLLVLH (SEQ ID NO: 41) and YT It is one or more amino acid sequences selected from AQGEPFPNNVEKLCAP (SEQ ID NO: 42), and the P1 and P2 peptide motifs bind to two or more different receptors on human induced pluripotent stem cells to activate cellular behavior of migration. It relates to an environmental structure, a three-dimensional surface for regulating the behavior of human induced pluripotent stem cells, an array, and a method for manufacturing a three-dimensional microenvironment structure.
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机译：本发明是一种纳米纤维膜;和P1（第一肽）和P2（第二肽）作为用于调节包含两个或更多个肽基序的人诱导的多能干细胞的行为的三维微环境结构，其中纳米纤维膜是聚偏二丙烯（PVDF），它包含它含有丙烯腈（锅），聚醚砜（PES）和聚乳酸（PLA）中的至少一种，其中P1（第一肽）基序与整合素α5β1结合为整联蛋白结合基序，pHSRN-它含有RGDSP的氨基酸序列（SEQ ID不：17），其中P2（第二肽）基序由毛毡细胞受体，转化生长因子（TGF）受体，成纤维细胞生长因子（FGF）受体和白血病抑制因子（LIF）受体组成。一种或多种整联蛋白α5β1共致受体结合基序选自基团（共接受接受者结合MO-TIF），以及与FRIZELD受体结合的P2（第二肽）基序是LCCGRGRTRTQRVTERCNC（SEQ ID NO：39）和LGTQGRLCNKTSEGMDGCEC（ SEQ ID NO：40）是选自的至少一种氨基酸序列，P2（第二肽）与TGF受体结合的P2（第二肽）是至少一种选自LTGKNFPMFHRN（SEQ ID NO：37）和MHRMPSFLPTT的氨基酸序列（SEQ ID NO：38），P2（第二肽）与FGF受体结合的基序是选自Anrylamkedglllas（SEQ ID NO：33），HFKDPKRLYCK（SEQ ID NO：34），FLPMSAK（SEQ ID）中选择的至少一种no：35）和Ktgpgqka（SEQ ID NO：36）氨基酸序列，与与LIF受体结合的P2（第二肽）基序是IVPLLLLVLH（SEQ ID NO：41）和YT是一种或多种氨基酸序列选自AQGEPFPNNVEVEKLCAP（SEQ ID NO：42），P1和P2肽基序与嗡嗡声上的两种或更多种不同的受体结合一种诱导多能干细胞，以激活迁移的细胞行为。它涉及一种环境结构，用于调节人诱导的多能干细胞，阵列的行为，阵列和制造三维微环境结构的方法的三维表面。
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