METHODS OF ISOLATING, CULTURING AND GENETIC ENGINEERING CELL POPULATIONS OF THE IMMUNE SYSTEM FOR ADOPTIVE THERAPY

摘要

FIELD: cell biology.;SUBSTANCE: disclosed is a method of enriching CD4+- and CD8+-T-cells, which involves conducting in a closed CD4+-cell selection system and sampling CD8+-cells from a sample containing primary human T-cells, and combining said cells at a desired ratio of about 10:1 to 1:10. Also described is a method for producing genetically engineered T-cells, where T-cells are obtained using said method.;EFFECT: this group of inventions can be used in producing cells for adoptive therapy.;64 cl, 2 dwg, 4 ex