PCDEXO-GS-SSB GENETIC CONSTRUCTS CONTAINING SEQUENCES ENCODING CHIMERIC SSB PROTEINS AND EXONUCLEASES OF THE BACTERIOPHAGE HOMOLOGOUS RECOMBINATION APPARATUS, COMBINED VIA A GLYCINE-SERINE POLYLINKER

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology, namely, to production of a recombinant SSB-GS-EXO protein construct. Proposed is a method for producing a recombinant SSB-GS-EXO protein construct, involving transformation of competent cells and expression of genetic constructs therein based on a plasmid DNA, wherein 5’-3'Flap exonuclease (EXO) and SSB protein are combined by genetic engineering through a glycine-serine spacer represented by the sequence SEQ ID NO: 1.;EFFECT: invention ensures cloning of DNA molecules with an increased frequency of homologous recombination between nucleic acids in the cell.;1 cl, 3 dwg, 1 tbl, 2 ex