A METHOD FOR PREPARING LYMPHOCYTE SAMPLE FOR FLOW CYTOMETRY ANALYSIS

摘要

The invention provides methods for preparing lymphocyte samples for flow cytometric analysis. The method includes: centrifuging anticoagulant blood samples to obtain blood cell precipitate, diluting the blood cell precipitate with PBS solution, then adding into a centrifuge tube containing lymphocyte separation solution, centrifuging, discarding the plasma to obtain buffy coat cells; then adding the buffy coat cells into a centrifuge tube, adding PBS solution, centrifuging, discarding the supernatant, adding PBS solution to adjust the concentration of cells, adding the cell solution into a flow tube; then adding antibody, mixing, incubating without light and centrifuging, discarding the supernatant and resuspending the cells in pre-cooled PBS-EDTA solution. The samples obtained enriches and purifies lymphocytes, fully preserves the activity of lymphocytes, removes granulocytes, red blood cells, platelets or other blood components, and the subpopulations to be detected are purified, the analysis and detection are more accurate, and costs for analyzing antibodies and reagents are saved.