METHOD AND USE FOR REAL-TIME FLUORESCENT PCR DETECTION OF CRONOBACTER SAKAZAKII O-ANTIGEN

摘要

A method and use for real-time fluorescent PCR detection of Cronobacter sakazakii O-antigen, primarily utilizing a real-time fluorescent PCR reaction rapid detection system of an MGB probe to detect seven O-antigen serotypes of Cronobacter sakazakii. With Cronobacter sakazakii O1-O2-type and O4-O6-type wzy genes and O3-type and O7-type wzx genes as target genes, a Taqman specific primer and the MGB probe directed at each serotype are designed and screened, and a real-time fluorescent PCR detection method is established. The detection method accurately, rapidly, and sensitively performs molecular biology detection of all seven Cronobacter sakazakii O-antigen serotype strains.