EXTRACELLULAR VESICLES (EVs) DERIVED FROM MESENCHYMAL STROMAL CELLS AND METHOD FOR OBTAINING SAID EVs

摘要

The current invention relates to a process for the manufacturing of a composition of extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs), said method comprises: culturing and expanding MSCs in a serum-free and xeno-free medium comprising purified human serum albumin and human transferrin; - collecting cell supernatant, said cell supernatant comprises EVs; - filtering said cell supernatant to obtain EVs; and concentrating said EVs, preferably by means of ultrafiltration. In a second and further aspect, said invention is directed to compositions comprising EVs and their clinical use.