Encapsulation of messenger RNA

摘要

PROBLEM TO BE SOLVED: To provide encapsulation of messenger RNA. The present invention provides an improved process for lipid nanoparticle formulations and mRNA encapsulation. In some embodiments, the invention is the process of encapsulating messenger RNA (mRNA) within lipid nanoparticles, comprising mixing the mRNA solution with the lipid solution, comprising the steps of mixing the mRNA solution and / or the lipid. The process is provided, wherein the solution is at a predetermined temperature above the ambient temperature. The present invention provides, among others, improved processes for lipid nanoparticle formulations and mRNA encapsulation. In particular, the present invention has significantly improved encapsulation efficiency, mRNA recovery, and more homogeneous and smaller particle size (eg, less than 100 nm) by preheating the mRNA and / or lipid solution prior to mixing. ) Is based on the amazing discovery that it brings. [Selection diagram] None