An amphipathic polyamino acid, a block copolymer using the amphipathic polyamino acid, and a complex containing the amphipathic polyamino acid or the block copolymer and nucleic acid.

摘要

According to the present invention, an amphipathic polyamino acid for nucleic acid delivery represented by the following formula (1) is provided.(During the ceremony, R 1 represents a hydroxyl group, an oxybenzyl group, an -O-R 1a group or an -NH-R 1b group, where R 1a and R 1b are independently unsubstituted or substituted linear or branched. Represents an alkyl group having 1 to 12 carbon atoms. R 2 is a hydrogen atom, an unsubstituted or substituted linear or branched alkyl group having 1 to 12 carbon atoms, or an unsubstituted or substituted linear or branched alkyl carbonyl group having 1 to 24 carbon atoms. Represent, R 3a , R 3b , R 4a and R 4b independently represent a methylene group or an ethylene group. R 5a and R 5b represent -O- or -NH- independently of each other. R 6a and R 6b represent unsubstituted or substituted aliphatic hydrocarbon groups having 7 to 12 carbon atoms, which may contain an alicyclic independently of each other. R 7a and R 7b are independent of each other and have the following groups: -NH- (CH 2 ) p1- [NH- (CH 2 ) q1- ] r1 NH 2 (i); −NH − (CH 2 ) p2 −N [− (CH 2 ) q2 −NH 2 ] 2 (ii); -NH- (CH2) p3 -N {[-(CH 2) q3 -NH 2][-(CH 2) q4 -NH-] r2 H} (iii); and -NH- (CH2) p4 - N {-(CH 2 ) q5- N [-(CH 2 ) q6 -NH 2 ] 2 } 2 (iv) Selected from the same or different groups of groups consisting of Here, p1 to p4, q1 to q6, and r1 to 2 are integers of 1 to 5 independently of each other. R 8 represents ornithine, lysine, homolysine, arginine, a side chain of an amino acid selected from the group consisting of homo arginine and histidine, m is an integer of 9 or more, n is an integer from 0 to m. x is an integer of 2 to 300 and y is an integer from 0 to x z is an integer from 0 to x However, the relation of y + z ≦ x and the relation of 11 ≦ m + x ≦ 400 are satisfied, and each repeating unit in the above formula (1) can exist at random. )