METHYLATION DETECTION METHOD AND KIT FOR BIOLOGICAL SAMPLE GENOMIC DNA OR FREE DNA MOLECULES

摘要

The present invention provides a methylation detection method and a kit for biological sample genomic DNA or free DNA molecules. The method comprises: obtaining transformed DNA molecules by means of a chemical reaction; performing, by using the transformed DNA molecules as a substrate, performing methylation-specific multiplex PCR reaction for one or more methylation regions of one or more genes by means of multiple pairs of methylation-specific primers, so as to obtain a PCR amplified product; and detecting methylation levels of different fragments in the amplified product.