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TIME-BASED CLUSTER IMAGING OF AMPLIFIED CONTIGUITY PRESERVED LIARARY FRAGMENTS OF GENOMIC DNA
TIME-BASED CLUSTER IMAGING OF AMPLIFIED CONTIGUITY PRESERVED LIARARY FRAGMENTS OF GENOMIC DNA
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机译:基于时间的扩增连续性的簇成像保存的基因组DNA的促序列碎片
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摘要
In an example method, a series of time-based clustering images is generated for a plurality of library fragments from a genome sample. Each time-based clustering image in the series is sequentially generated. To generate each time-based clustering image in the series: i) a respective sample is introduced to a flow cell, the respective sample including contiguity preserved library fragments of the plurality of library fragments, wherein the contiguity preserved library fragments are attached to a solid support or are attached to each other; ii) the contiguity preserved library fragments are released from the solid support or from each other; iii) the contiguity preserved library fragments are amplified to generate a plurality of respective template strands; iv) the respective template strands are stained; and v) the respective template strands are imaged.
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