SCREENING, IDENTIFICATION, AND OPTIMIZED EXPRESSION OF CHONDROSULFATASE

摘要

The invention belongs to the technical field of biological engineering, and discloses the screening, identification and optimized expression of chondrosulphatase. The invention screens and identifies a chondrosulphatase from the nature, which the highest similarity between its amino acid sequence and the chondrosulphatase reported by NCBI is 85%. The invention achieved the high-efficiency biosynthesis of high activity chondrosulphatase with the highest enzyme activity of 11976.5 U/L by expression optimization in E. coli and B. subtilis, and simplified the whole process and post-treatment. The invention has potential and wide application value in the preparation of products containing low molecular weight chondrosulphatase in the fields of medicine, cosmetics and biology, and lays a foundation for the efficient fermentation of microbial system to prepare chondrosulphatase with high enzyme activity, and is suitable for industrial production and application.