A DC offset calibration system and method. The method includes: in a first calibration mode, outputting a first digital signal by using a control circuit, generating a first differential calibration signal according to the first digital signal by using a first digital-to-analog conversion circuit, generating a first amplified signal according to the first differential calibration signal by using an amplification circuit, and feeding back the first amplified signal to the control circuit to adjust the first digital signal; and in a second calibration mode, outputting a second digital signal by using the control circuit, generating a second differential calibration signal according to the second digital signal by using a second digital-to-analog conversion circuit, generating a second amplified signal according to the second digital signal by using an equalizing circuit and the amplification circuit, and feeding back the second amplified signal to the control circuit to adjust the second digital signal.
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