MULTI-FUNCTIONAL GSH-RESPONSIVE SILICA NANOPARTICLES FOR DELIVERY OF BIOMOLECULES INTO PLANT CELLS

摘要

The present technology provides a nanoparticle that includes a silica network comprising crosslinked polysiloxanes, wherein the crosslinks comprise disulfide linkages, and the nanoparticle has a surface bearing charged functional groups and a surface potential of either less than -30 mV or greater than +30 mV, and wherein the nanoparticle has an average diameter of 20 nm to 60 nm. The nanoparticles may be used to efficiently deliver biomolecules to plant cells, including polynucleic acids, proteins and complexes threrof (e.g., Cas9 RNP).